bodipy cholesterol invitrogen

Low-Density Lipoprotein Uptake Inhibits the Activation and Antitumor ... Many fluorescent lipid probes tend to loop back to the membrane interface when attached to a lipid acyl chain rather than embedding deeply into the bilayer. CholEsteryl 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-<i>s</i ... ment with cholesterol-lowering drugs, 3 LDL apheresis, 4 and orthotopic liver transplantation,5 all of which have substan-tial limitations. 荧光显微镜技术的基本原理是借助荧光剂让细胞成分呈现高度具体的可视化效果，比如在目的蛋白后面连一个通用的荧光蛋白—GFP。. Analysis of intracellular BODIPY-cholesterol efflux. After cholesterol overload, the effect is analogous to that which has been reported in the case of Niemann-Pick type C (NPC) fibroblasts, where lac-Cer accumulates aberrantly in . Y3603) from Molecular Probes . We also show that over-expression of human Patched in the yeast S. cerevisiae results in a significant boost of BODIPY-cholesterol efflux. BODIPY-cholesterol was quantified by flow cytometry. Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. Changes in the targeting of BODIPY-lac-Cer under cholesterol perturbations may reflect a response of the cells to perceived nutritional state, causing a rerouting . The cells were mounted with medium containing 4',6-diamidino-2-phénylindole (DAPI, blue), analyzed under confocal microscope, and imaged. Cells incubated with only CholEsteryl BODIPY™, at an equivalent concentration to 60 µg/mL of NPs, were used as control and imaged at 24 h. Insert 90× magnification. BODIPY 493/503 Staining of Neutral Lipid Droplets for ... - PubMed We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult . . Many fluorescent lipid probes tend to loop back to the membrane interface when attached to a lipid acyl chain rather than embedding deeply into the bilayer. New BODIPY lipid probes for fluorescence studies of membranes Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. . . Subjective motility was evaluated with light microscopy following dilution. Western blotting was done using precast mini gels (Invitrogen) and probed using a polyclonal hLDLR antibody. In this study, the validated BODIPY-cholesterol assay was used for the first time in ram spermatozoa to quantify cholesterol efflux by tracking the loss of BODIPY-cholesterol from the sperm plasma membrane .

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